Barry Sharp Lester invented "Click chemistry." He won the 2022 Nobel. Click chemistry has many advantages. Click chemistry has shaped several fields of contemporary chemistry since its inception. Click chemistry has helped us understand and prepare complex molecules. This review examines Click response importance. The Huisgen 1,3-dipolar cycloaddition technique produces 1,4-disubstituted 1,2,3-triazoles from azode and terminal alkyne. This discipline is also rapidly expanding in research and development. This paper provides a quick review of CC and its many uses. CuI frequently employs azide-alkyne cycloaddition since it is a powerful change agent. According to research and review studies, generation-wise Click chemistry with the Cu(I) catalyst facilitates coupling processes. Click reaction types and groups can be formed from alkynes, cyclooctynes, and azides. This study covers Click chemistry and its applications in drug discovery, Click chemistry-assisted steroid substitution, the Metal-Free Click Reaction, drug production, and three-dimensional bio-printing. We also examined Click chemistry's pros and cons and advantages.

UV absorption coating is an important function material, which can protect the substrates from photoaging. In this work, a class of sustainable UV-absorbent copolymers derived from ethyl cellulose (EC), fatty acid and rosin were prepared by a combination of atom transfer radical polymerization (ATRP) and Click chemistry. To fulfill this strategy, the pendant azides were first attached onto the backbone of EC. Then, ATRP was applied to fabricate well-defined poly(lauryl methacrylate) (PLMA) bearing terminal alkynes. Finally, Click chemistry between the pendant azides in EC and the alkynes in PLMA as well as in rosin esters (DAPE), was performed to achieve the cellulose-rosin graft copolymers (EC-(g-DAPE)-g-PLMA) with UV absorption property. The chemical structure of cellulose-rosin graft copolymers was confirmed by FTIR, 1H NMR and GPC. Thermodynamic performance analysis indicated that these EC-rosin graft copolymers showed better thermal stability than EC. Due to the synergistic hydrophobic interaction of rosin and the hydrophobic lauryl groups in PLMA, these graft copolymers showed excellent hydrophobic property, and the static contact angles were all above 90°, . In addition, all the EC-rosin graft copolymers showed outstanding and stable UV absorption capability, and maintained excellent UV absorption capability after continuous UV-irradiation for 1 h or being heated to 100 °, C for 0. 5 h, which had potential application in UV absorption materials.

In situ preparation and functionalization of poly(vinyl chloride) (PVC) nanofibers were investigated by a combination of copper(I)-catalyzed azide alkyne cycloaddition (CuAAC) Click chemistry and electrospinning methods. For CuAAC Click reaction, the azido groups were installed on the PVC via nucleophilic substitution reaction with sodium azide. Various experimental conditions including concentration of Clickable compounds, applied voltage, collector charge and feeding speed were intensively investigated by both spectral and microscopic analyses. The efficiency of CuAAC Click reactions was monitored by Fourier transform infrared spectroscopy (FTIR) spectroscopy through the decrease of the azide band of the azido-functionalized PVC in its spectrum. The increase on phenylacetylene concentration from one-to twofold as Clickable compound, applied voltage from 25 to 35 kV and the decrease feeding speed from 0. 5 to 0. 1 mL/h gradually improved the CuAAC Click efficiency between azido-functionalized PVC (PVC-N3) and phenylacetylene (PA). The best CuAAC Click efficiency was found as 70% with optimized condition (6: 2: 0. 01 , = , azide-functionalized PVC: phenylacetylene: copper(II) sulfate, 35 kV voltage, negatively charged collector and 0. 1 mL/h feeding speed). In addition, the proton nuclear magnetic resonance (1H NMR) spectroscopy confirmed the chemical structure of the Clicked product of PVC-N3 and phenylacetylene (PA) and CuAAC Click efficiency was calculated from the corresponding peaks as 69. 2%. The diameters of nanofibers produced by either electrically positive or negative charged collectors were also investigated by scanning electron microscopy and their diameter distributions were found as 10–, 370 and 20–, 400 nm, respectively. The in situ functionalization of PVC-N3 led to obtain improved thermal properties of the Clicked product based on differential scanning calorimeter (DSC).

Aim and Background: This study investigates the biological properties of cyclic RGD peptides being covalently immobilized via Click chemistry onto alkyne terminated silicon surfaces. Material and methods: At first cyclic RGD peptides were reacted with 4-azidophenyl isothiocyanate via a specific reaction. The azidated peptide was then covalently immobilized on an alkyne-terminated monolayer on silicon surfaces via the Cu (I)-catalyzed 1, 3-dipolarcycloaddition reaction. The surface structures of the attached peptide and control surfaces were characterized using X-ray photoelectron spectroscopy (XPS) and Fourier Transform Infrared (ATR-FTIR) Spectroscopy. Then after seeding human fibroblast cells on the peptide and control substrates, Scanning electron microscopy (SEM) and Laser scanning confocal microscopy (LSCM) was used to examine the morphology of human fibroblast cells. Adhesion and proliferation of human fibroblast cells on control and modified surfaces were analyzed by cell adhesion and (MTS) assay. Results: XPS and ATR-FTIR analysis showed the cyclic RGD peptides were successfully immobilized on silicon surfaces. Scanning electron microscopy (SEM) and Laser scanning confocal microscopy (LSCM) demonstrated that human fibroblast cells homogenously distributed across the peptide-modified substrates and had a uniform appearance. Peptide-modified surfaces exhibited substantially superior cellular responses compared to those on control surfaces. Conclusion: The research may suggest a procedure for the fabrication of biologically active surfaces in biosensor platforms.